Humanin (HN)
Humanin (HN) is a high-purity, synthetic 24-amino acid peptide originally discovered in the surviving brain cells of Alzheimer’s patients. As the founding member of the Mitochondrial-Derived Peptides (MDPs) family, Humanin is encoded by the 16S rRNA gene within the mitochondrial genome. In the landscape of advanced molecular biology and aging research, Humanin is highly distinguished by its potent cytoprotective and anti-apoptotic properties. It functions as a retrograde signaling molecule, acting both intracellularly and extracellularly to protect cells against a variety of stressors, including oxidative stress, amyloid-beta toxicity, and hypoxic injury. It provides researchers with a robust experimental model to investigate mitochondrial-nuclear communication, insulin sensitivity pathways, and the systemic regulation of cellular longevity.
Our Humanin is synthesized in an ISO 9001:2015 certified environment utilizing high-precision Solid-Phase Peptide Synthesis (SPPS). We achieve an ultra-high chemical purity of ≥99%, verified by HPLC and Mass Spectrometry (MS). This rigorous production standard ensures the peptide is free from truncated sequences and residual chemical reagents, guaranteeing the consistency and structural fidelity required for high-stakes studies into neuroprotection and metabolic homeostasis.
Research Applications & Mechanisms
Neuroprotection & Alzheimer’s Models
Investigating the ability of Humanin to neutralize Amyloid-beta (Aβ) toxicity and protect neuronal cells from apoptosis in experimental neurodegeneration research.
Anti-Apoptotic Signaling Pathways
Researching the interaction of Humanin with the pro-apoptotic protein Bax, inhibiting its translocation to the mitochondria and maintaining cellular membrane integrity.
Metabolic Sensitivity & Glucose Homeostasis
Studying the peptide’s role in enhancing insulin sensitivity and modulating the metabolic rate through the STAT3 signaling pathway in research models of metabolic syndrome.
Cardiovascular & Endothelial Stability
Exploring the influence of Humanin on vascular endothelial cells to research the mitigation of oxidative stress-induced damage in cardiovascular tissue models.
Technical Specifications
| Parameter | Laboratory Standard |
| Compound | Humanin (HN) |
| Sequence | Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala |
| Purity (HPLC) | ≥99% (Pharmaceutical Grade) |
| Physical Appearance | Fine, Smooth & Flat Lyophilized Cake |
| Molecular Weight | 2687.2 Da |
| Concentration | 10mg per vial |
| Solubility | Soluble in Water / PBS / Bacteriostatic Water |
| Batch Control | MS & HPLC Analysis Verified |
Key Features & Quality Assurance
Pharmaceutical-Grade Lyophilization
Our proprietary vacuum-sealing process ensures the Humanin powder surface is perfectly smooth, flat, and delicate. This uniform “cake” structure ensures rapid, clear reconstitution and maintains the molecular stability of the 24-amino acid sequence during long-term storage.
High-Fidelity Sequence Integrity
Every batch undergoes strict Mass Spectrometry (MS) to confirm the exact amino acid sequence, ensuring maximal receptor binding affinity and reproducible results in complex cellular assays.
Reconstitution & Storage
Reconstitution Guide
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Preparation: Allow the vial to reach room temperature. Centrifuge for 30-60 seconds to ensure the Humanin cake is at the base.
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Solvent: Reconstitute with Sterile Water or PBS.
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Technique: Gently introduce the diluent against the vial wall. DO NOT SHAKE; swirl gently until the solution is clear and transparent.
Storage Protocols
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Long-term: Store at -20°C (Maintains potency for up to 24 months).
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Short-term: Stable at 2–8°C for up to 3 months.
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Post-Reconstitution: Store at 2–8°C and utilize within 10-14 days for optimal results.
Shipping & Documentation
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Global Logistics: Shipped in discreet, temperature-controlled packaging to maintain the structural integrity of the Humanin peptide.
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Verified Quality: A batch-specific COA and HPLC/MS reports are available for every order.
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Reshipment Policy: We provide a guaranteed reshipment policy for any lost or customs-intercepted orders (terms apply).
⚠️ Research Disclaimer
Product-Specific Safety Notice: This Humanin (HN) is strictly for laboratory research and analytical purposes. It is NOT intended for human or veterinary administration. All research must be conducted by qualified professionals.
Scientific Resources & References
- Structural, sequence, and mitochondrial-derived peptide data for Humanin (HN) can be accessed via UniProt.
- Comprehensive chemical and bioactivity information for Humanin (HN), including experimental research context, can be explored through PubChem.
🔍 Deep Dive Research
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[Technical Guide]: How to Reconstitute and Store Lyophilized Peptides for Maximum Potency
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[Mitochondria Study]: The Retrograde Signal: Exploring Humanin in Modern Neuroprotection Research
jamieheaton –
Will reorder soon.